LOCUS       sequence-100462-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: RFP and
            blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-RB
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..123
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             124..522
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAMPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(622..641)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    687..742
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(741..760)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagggc
       61 agtggagcta ctaacttcag cctgctgaag caggctggtg acgtcgagga gaatcctggc
      121 cccatggcca tgcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
      181 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
      241 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
      301 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
      361 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
      421 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
      481 attcgtgaat tgctgccctc tggttatgtg tgggagggct aattcgaagg cctgtcgtga
      541 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
      601 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
      661 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
      721 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
      781 tcagaatgag ta
//
