LOCUS       sequence-100465-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of GFP, rtTA and 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-GP
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cttgcgcagc ttgcaagact ataagattca atctgccctg ctggtgccca cactatttag
       61 cttcttcgct aagagcactc tcatcgacaa gtacgaccta agcaacttgc acgagatcgc
      121 cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac gcttccacct
      181 accaggcatc cgccagggct acggcctgac agaaacaacc agcgccattc tgatcacccc
      241 cgaaggggac gacaagcctg gcgcagtagg caaggtggtg cccttcttcg aggctaaggt
      301 ggtggacttg gacaccggta agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg
      361 tggccccatg atcatgagcg gctacgttaa caaccccgag gctacaaacg ctctcatcga
      421 caaggacggc tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt
      481 catcgtggac cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga
      541 actggagagc atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc
      601 cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat
      661 gaccgagaag gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg
      721 cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg
      781 caagatccgc gagattctca ttaaggccaa gaagggcggc aagatcgccg tgtaaggcct
      841 gtcaggccaa gct
//
