LOCUS       sequence-100468-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of BFP, rtTA and 
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-BH
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg ccctgctggt
       61 gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg acctaagcaa
      121 cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc
      181 caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa caaccagcgc
      241 cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg tggtgccctt
      301 cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga accagcgcgg
      361 cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc ccgaggctac
      421 aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct actgggacga
      481 ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca agggctacca
      541 ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct tcgacgccgg
      601 ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga
      661 acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc aggttacaac
      721 cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg
      781 caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg gcggcaagat
      841 cgccgtgtaa ggcctgtcag gccaagct
//
