LOCUS       sequence-100471-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: 
            neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-Neo
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc
       61 ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg
      121 ttcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc
      181 actgacgggc accggagcct ctaggcgaga ccctgtctca caaaataaag taagcccgga
      241 ctgagtgcgg aaaggcgggc ctggcgggtc tggtctcccc atgcgggcca ccagaggccc
      301 tgcagccttc agtcgcttga aggggtaatg gcgcttccac tcacaaacat ggcggacaga
      361 gcgtgtgaac gagatgaaca gcccctcaaa aatatggccg ccgaggctgg acggccgtgc
      421 cccagcagca ccgcctccgc gccccacgtg atctctcgcc gggcacagcg ctgaccgcgg
      481 aggtccaacc ggaagaatgt ccggattgga cattcggaag agggcccgcc ttccctgggg
      541 aatctctgcg cacgcgcaga acgcttcgac caatgaaaac acaggaagcc gtccgcgcaa
      601 ccgcgttgcg tcacttctgc cgcccctgtt tcaaggtata tagccgtaga cggaacttcg
      661 cctttctctc ggccttagcg ccattttttt gggtgagtgt tttttggttc ctgcgttggg
      721 attccgtgta caatccatag acatctgacc tcggcactta gcatcatcac agcaaactaa
      781 ctgtagcctt tctctctttc cctgtagaaa cctctgcacc tgaggccacc atgattgaac
      841 aagatggatt gcacgcag
//
