LOCUS       sequence-100472-       12263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for trans-Golgi network mCherry-MtSYP41 
            expressed under AtUBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12263)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 12263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMU-TGN41r
FEATURES             Location/Qualifiers
     source          1..12263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             290..1081
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1325..1913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1814..1833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2067..2084
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2099..2239
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2165..2184)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2325..2347
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2583..2777
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2843..3910)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(4344..4973)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6273..6297
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6367..6389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6386..6402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6386..6402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    6422..6442
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8429..8453
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8456..9163
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     8869..8888
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(9165..9189)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10170..10190)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10198..10215)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10198..10214)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10207..10229)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     regulatory      10256..10265
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        10609..10788
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             10822..11616
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10876..10895)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11486..11505
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      11656..11908
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    12001..12025
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 attcgggcac gaacccagtg gacataagcc tcgttcggtt cgtaagctgt aatgcaagta
       61 gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      121 gcagtggcgg ttttcatggc ttcttgttat gacatgtttt tttggggtac agtctatgcc
      181 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      241 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
      301 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      361 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      421 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
      481 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
      541 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
      601 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
      661 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
      721 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      781 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
      841 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
      901 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
      961 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1021 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     1081 atgtctagct agaaattcgt tcaagccgac gccgcttcgc cggcgttaac tcaagcgatt
     1141 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     1201 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     2221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     2401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     2461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     2521 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     2581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     2641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     2701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     2761 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     2821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     2881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     2941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     3001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     3061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     3121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     3181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     3241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     3301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     3361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     3421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     3481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     3541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     3601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     3661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     3721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     3781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     3841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     3901 gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     3961 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     4021 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     4081 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     4141 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     4201 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     4261 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     4321 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     4381 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     4441 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     4501 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     4561 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     4621 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     4681 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     4741 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     4801 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     4861 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     4921 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     4981 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     5041 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     5101 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     5161 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     5221 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
     5281 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
     5341 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
     5401 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
     5461 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
     5521 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
     5581 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
     5641 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
     5701 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
     5761 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
     5821 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
     5881 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
     5941 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
     6001 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
     6061 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
     6121 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
     6181 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     6241 ccgttattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
     6301 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg
     6361 ggcccgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta
     6421 tcaactttgt atagaaaagt tgagtctagc tcaacagagc ttttaaccca aattggtaca
     6481 atagaataca actttagatc ataattctca aaagaaagag attccttagc tattctatct
     6541 gccactccat ttccttctcg gcttgtatgc acaagcataa aatcctcaaa cttgctaagt
     6601 agatacttta tgtcttggat aattggattg agacttgaca agcataactt tcatgtaacc
     6661 aaagacacaa gttgctgaga atccacctca aaaatgatct tcctataatt gaatcgggat
     6721 aatgacagca cagcccatct aagagcctcc acttctactt ccagcacgct tcttactttt
     6781 accacagctc ttgcacctaa ccataacacc ttccctgtat gatcgcgaag cacccaccct
     6841 aagccacatt ttaatccttc tgttggccat gccccatcaa agttgcactt aacccaagat
     6901 tgtggtggag cttcccatgt ttctcgtctg tcccgacggt gttgtggttg gtgctttcct
     6961 tacattctga gcctctttcc ttctaatcca ctcatctgca tcttcttgtg tccttactaa
     7021 tacctcattg gttccaaatt ccctcccttt aagcaccagc tcgtttctgt tcttccacag
     7081 cctcccaagt atccaaggga ctaaagcctc cacattcttc agatcaggat attcttgttt
     7141 aagatgttga actctatgga ggtttgtatg aactgatgat ctaggaccgg ataagttccc
     7201 ttcttcatag cgaacttatt caaagaatgt tttgtgtatc attcttgtta cattgttatt
     7261 aatgaaaaaa tattattggt cattggactg aacacgagtg ttaaatatgg accaggcccc
     7321 aaataagatc cattgatata tgaattaaat aacaagaata aatcgagtca ccaaaccact
     7381 tgcctttttt aacgagactt gttcaccaac ttgatacaaa agtcattatc ctatgcaaat
     7441 caataatcat acaaaaatat ccaataacac taaaaaatta aaagaaatgg ataatttcac
     7501 aatatgttat acgataaaga agttactttt ccaagaaatt cactgatttt ataagcccac
     7561 ttgcattaga taaatggcaa aaaaaaacaa aaaggaaaag aaataaagca cgaagaattc
     7621 tagaaaatac gaaatacgct tcaatgcagt gggacccacg gttcaattat tgccaatttt
     7681 cagctccacc gtatatttaa aaaataaaac gataatgcta aaaaaatata aatcgtaacg
     7741 atcgttaaat ctcaacggct ggatcttatg acgaccgtta gaaattgtgg ttgtcgacga
     7801 gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt gtttatcaac
     7861 tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa aacaactttg
     7921 cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt caccgcctta
     7981 gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa aacaataccc
     8041 aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa aaactttctc
     8101 tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct caaaatcttc
     8161 gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat tctgttaatc
     8221 ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt ctcgattagg
     8281 gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat aattactctt
     8341 cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat cgaatttgtc
     8401 gattaatctg agtttttctg attaacaggc aagtttgtac aaaaaagcag gcttcatggt
     8461 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     8521 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     8581 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     8641 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     8701 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     8761 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     8821 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     8881 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     8941 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     9001 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     9061 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     9121 cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggacccag ctttcttgta
     9181 caaagtggaa atggcgacga ggaatcgcac tttgattttc cgaaaataca gagatgcatt
     9241 gaaaagtgtc cgtgctccgt cgtcatcttc gccgccgtcc acctcctccc gcggtggtcc
     9301 ggtaatcgaa ttggtcagca cttcgcttct taatcccaat cgctcttatg ctccgcttag
     9361 tactgatgat cctggtggtt caagtaaggg tttaaatcca attactgtgg ggcttcctcc
     9421 agcatgggtg gatgtatctg aagaaatatc agcaaatgtg caacgtgcac gcacaaaaat
     9481 ggcagaactg tccaaggctc atgcaaaggc cttaatgcca tcgtttggag atggtaagga
     9541 cgaccaacat gctattgagt ctctaacaca cgaggtaact gacttgataa agagatcgga
     9601 gaagaggctg cggagacttg cttctgcagg gccttctgag gattctaatg tcaggaaaaa
     9661 tgtgcagcgt tctcttgcta ctgaccttca gagcctctct gtagatcttc gcaagaaaca
     9721 atcaacttat ttgaagcgcc tcagacagca aaaagagggt cacgatgggg ttgacctaga
     9781 gatcaacatg aatggaagta aatctaaata tgaagacgat gacttggaca atatgatatt
     9841 taacgagcat cagatggcca agctaaaaaa aagcgaagct ttcacagttg aaagagaaaa
     9901 agagatccaa caggttgttg aatctgttaa cgagcttgct cagattatga aggatttatc
     9961 agtactagtc atagaccagg gaaccattgt tgatagaata gactacaaca ttcagaatgt
    10021 ggcaaccacg gttgaggatg gccttaaaca attgcagaag gcagagagaa ctcagaagaa
    10081 gggaggcatg gtaatgtgtg cgtcagtgct tcttatcatg tgctttgtta tgttggttct
    10141 cttaatcatt aaggaaatta ttttgtgagc aactttatta tacatagttg ataattcact
    10201 ggccgtcgtt ttacaacgtc gtgactggga aaacgatcta actgactagc cgcggccatg
    10261 gcggccggga gcggccatgc tagagtccgc aaaaatcacc agtctctctc tacaaatcta
    10321 tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt
    10381 tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
    10441 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacctgca
    10501 ggtcgaccat atgggagagc tcgaattatc atacatgaga attaagggag tcacgttatg
    10561 acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt
    10621 tgaaggagcc actcagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa
    10681 accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt
    10741 caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt
    10801 cactctcaac tcgatcgagg catgattgaa caagatggat tgcacgcagg ttctccggcc
    10861 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
    10921 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
    10981 tccggtgccc tgaatgaact ccaagacgag gcagcgcggc tatcgtggct ggccacgacg
    11041 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
    11101 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
    11161 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
    11221 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
    11281 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
    11341 ctcaaggcgc ggatgcccga cggcgaggat ctcgtcgtga cccacggcga tgcctgcttg
    11401 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
    11461 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
    11521 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
    11581 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcggactct
    11641 agctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
    11701 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
    11761 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
    11821 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
    11881 cgcgcggtgt catctatgtt actagatcga ccggcatgca agctgataat tcaattcggc
    11941 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
    12001 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
    12061 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
    12121 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
    12181 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
    12241 cagagcgttg ctgcctgtga tca
//
