LOCUS       sequence-100477-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-Pur
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(91..302)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tgcagccagg ggcgtggaag taattcaagg cacgcaaggg ccataacccg taaagaggcc
       61 aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc cgttgcgaaa
      121 aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag
      181 gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc
      241 ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc
      301 ccactgacgg gcaccggagc ctctaggcga gaccctgtct cacaaaataa agtaagcccg
      361 gactgagtgc ggaaaggcgg gcctggcggg tctggtctcc ccatgcgggc caccagaggc
      421 cctgcagcct tcagtcgctt gaaggggtaa tggcgcttcc actcacaaac atggcggaca
      481 gagcgtgtga acgagatgaa cagcccctca aaaatatggc cgccgaggct ggacggccgt
      541 gccccagcag caccgcctcc gcgccccacg tgatctctcg ccgggcacag cgctgaccgc
      601 ggaggtccaa ccggaagaat gtccggattg gacattcgga agagggcccg ccttccctgg
      661 ggaatctctg cgcacgcgca gaacgcttcg accaatgaaa acacaggaag ccgtccgcgc
      721 aaccgcgttg cgtcacttct gccgcccctg tttcaaggta tatagccgta gacggaactt
      781 cgcctttctc tcggccttag cgccattttt ttgggtgagt gttttttggt tcctgcgttg
      841 ggattccgtg tacaatccat agacatctga cctcggcact tagcatcatc acagcaaact
      901 aactgtagcc tttctctctt
//
