LOCUS       sequence-100481-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of RFP, rtTA and 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-RP
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga
       61 ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac
      121 actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca
      181 cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg
      241 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct
      301 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga
      361 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct
      421 gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc
      481 tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga
      541 gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc
      601 cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc
      661 cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg
      721 taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa
      781 gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt
      841 ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt
      901 gtaaggcc
//
