LOCUS       sequence-100487-       10519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under 
            MtBCP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10519)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 10519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMB-APr
FEATURES             Location/Qualifiers
     source          1..10519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(648..1442)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(759..778)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1369..1388
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1476..1655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1773..1795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1792..1808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1792..1808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1828..1848
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3030..3054
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3126..3833
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3539..3558
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3835..3859)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4090..4110)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4118..4135)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4118..4134)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4127..4149)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4223..4247
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5547..6176
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6610..7677
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7743..7937
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8173..8195)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8281..8421
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8336..8355
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8436..8453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8607..9195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8687..8706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9439..10230)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcatg tatgataatt
     1741 cgagggtacc cggggatcct ctagagggcc cgagcggata acaatttcac acaggaaaca
     1801 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgga gagagggaga
     1861 tgtgttttta aggagctata gaaggataca ataccgcttc tttgattggt attctaactc
     1921 aatgcgttgc ttaagagttg gttaatctat ttgaaatgtt gcgattatag tagatcttgt
     1981 gctcccatct ttaatatatc ttttacactt actccttagt tatacaaaca ttaaacatca
     2041 taacttctaa ggtaattgat acgtgccaaa ttttctaaac tccattaaat ttggtttgaa
     2101 agaaatttca tgaaattcct ctagttcgtt ataaatatta gataattagg ttttgagtag
     2161 cattgtagct accatgacct atttccatta catttgtagg tttaaaagat caaagatgac
     2221 ttggggtatc aattaacgag tgagcatata tgcgagcaag gaaagagata agtcagagtg
     2281 tttaaggaaa gcaaggcaag aaaattcact tagggagcca aaacaaggtc aatactcgat
     2341 tacatctcta atttaagctt gagaacttga tatattcgag actcctcaag tatttgtact
     2401 ccaagtgtca atactcgatt acatctctaa ttttccaaat aaagaaactc gtaagaaaca
     2461 aacattattt atgtgtaaac tttctgcatg gagacaactc aattataacg aagcccggaa
     2521 gaggaactat atatactgtt tctatttccc tttgacacgg tccacactat attgcatact
     2581 tgtttactgc caaataaaaa caagtatgtc cacgtggtta aataaaaaca aaagaaaact
     2641 ttctttttgt tcaagtgata gaaagtttct ttacattaat ataaaataat ataagtgtat
     2701 tccaaaaagg taaaaaaaaa aataataagt tgtggaaatt caacctcgtc ttagtaaaaa
     2761 gccgaccaaa tccggtagcc gtttataata gcaaacaaat attctattat tgttgtgacg
     2821 ataaaatcaa tacacccaca catgacaact atatatagcc taagttgata tgcacaattc
     2881 atcaaatcaa atcaaaacaa ttacatttgc taacttgcta ttcctttgat tgagatttta
     2941 ttctaggaaa taaaattttg acactcattt ttttaaagtt aatttctaca tttagagaga
     3001 aatattttcc aaaatcaaat tttgatcaag caagtttgta caaaaaagca ggcttcatgg
     3061 cttcttctcg tgtagttttg atcctttcca tttccatggt tttactttca tcagttgcaa
     3121 ttgcaatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     3181 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     3241 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     3301 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     3361 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     3421 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     3481 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     3541 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     3601 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     3661 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     3721 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     3781 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggacccag
     3841 ctttcttgta caaagtggaa taagcggcca tgctagagtc cgcaaaaatc accagtctct
     3901 ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata
     3961 agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
     4021 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     4081 ccagtgacgc aactttatta tacatagttg ataattcact ggccgtcgtt ttacaacgtc
     4141 gtgactggga aaacgagctc aagcttagct tgagcttgga tcagattgtc gtttcccgcc
     4201 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     4261 gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
     4321 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
     4381 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat
     4441 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
     4501 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
     4561 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
     4621 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
     4681 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
     4741 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
     4801 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
     4861 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
     4921 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
     4981 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
     5041 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
     5101 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
     5161 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
     5221 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
     5281 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
     5341 gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
     5401 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
     5461 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
     5521 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
     5581 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
     5641 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
     5701 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
     5761 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
     5821 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
     5881 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
     5941 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
     6001 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
     6061 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
     6121 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
     6181 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
     6241 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     6301 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     6361 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
     6421 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     6481 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     6541 taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     6601 cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
     6661 ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca
     6721 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
     6781 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
     6841 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
     6901 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
     6961 gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac
     7021 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
     7081 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
     7141 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
     7201 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
     7261 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
     7321 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
     7381 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
     7441 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
     7501 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
     7561 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
     7621 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
     7681 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt
     7741 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
     7801 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
     7861 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
     7921 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
     7981 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
     8041 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
     8101 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
     8161 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     8221 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     8281 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     8341 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     8401 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8941 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     9061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9121 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9181 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9241 gaaaactcac gttaagggat tttggtcatg catgatatat ctcccaattt gtgtagggct
     9301 tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa
     9361 ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg cgaagcggcg tcggcttgaa
     9421 cgaatttcta gctagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
     9481 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
     9541 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
     9601 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
     9661 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
     9721 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
     9781 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
     9841 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
     9901 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
     9961 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
    10021 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
    10081 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
    10141 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
    10201 cgatacttcg gcgatcaccg cttcccccat gatgtttaac tttgttttag ggcgactgcc
    10261 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
    10321 ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacatgtca taacaagaag
    10381 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
    10441 tgcgtgacgg cagttacgct acttgcatta cagcttacga accgaacgag gcttatgtcc
    10501 actgggttcg tgcccgaat
//
