LOCUS       sequence-100490-       10203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under 
            MtPT4 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMP-APr
FEATURES             Location/Qualifiers
     source          1..10203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(648..1442)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(759..778)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1369..1388
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1476..1655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1773..1795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1792..1808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1792..1808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1828..1848
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2714..2738
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2810..3517
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3223..3242
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3519..3543)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3774..3794)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3802..3819)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3802..3818)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3811..3833)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3907..3931
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5231..5860
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6294..7361
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7427..7621
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7857..7879)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7965..8105
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8020..8039
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8120..8137)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8291..8879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8371..8390)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9123..9914)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcatg tatgataatt
     1741 cgagggtacc cggggatcct ctagagggcc cgagcggata acaatttcac acaggaaaca
     1801 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgga ctcgatccac
     1861 aacaaagatt aatttttgtt caaacaacta atactatctg aaaatacact tccaaaaata
     1921 tgttaaattt ttgttttttt taacatggag tgaaaaaaag ttgctccctt attatttaat
     1981 tggcacacat ttttgtatgt ttagtaaatg acacgcattc tattcggtgg taacgtttgt
     2041 cctcacatac acctcatttg aattgcattt gtatcctact acctcccttt ccaattatat
     2101 aagatatttt tgaatttttt ttgttccttt ttataagacc tccttgttat ttccaattaa
     2161 attaattatt tctttacata tatgtcccta tttattatac atttttttct tcaatcagca
     2221 ataaatagca tgttgaaaat ataaactatc tctctttcta aaggataaaa ttgtaaaatc
     2281 aatagtgatt acaaataact ttaatacaaa tactcacttt cttaattcct gtgattttgg
     2341 caaaaaggtc ttgtatatag ggacggaggt agtattatgt tagcaagcag ctaaaaaaca
     2401 aaacaataat cctcttttaa cagctaactg taaccaaaac aaaagcactt agcatttttg
     2461 aacattccct ataagaatat cctaattaat tgatcaacac taggtgaata tccataacat
     2521 ttagctagca atctcaattt ttcttgttct catctcctaa gctaacttag gactaaacgt
     2581 cacattgtta cattatcaaa cacatatcaa tgcatagcca taataatata tataagagca
     2641 tacacataaa cacctaaacc aaattaactc caaaaaccaa cttgagagag tcttcctttt
     2701 tttttttacc aaagcaagtt tgtacaaaaa agcaggcttc atggcttctt ctcgtgtagt
     2761 tttgatcctt tccatttcca tggttttact ttcatcagtt gcaattgcaa tggtgagcaa
     2821 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     2881 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     2941 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     3001 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     3061 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     3121 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     3181 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     3241 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     3301 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     3361 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     3421 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     3481 ccgccactcc accggcggca tggacgagct gtacaaggac ccagctttct tgtacaaagt
     3541 ggaataagcg gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct
     3601 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
     3661 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     3721 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg acgcaacttt
     3781 attatacata gttgataatt cactggccgt cgttttacaa cgtcgtgact gggaaaacga
     3841 gctcaagctt agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc
     3901 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     3961 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     4021 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     4081 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
     4141 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     4201 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     4261 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     4321 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     4381 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     4441 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     4501 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     4561 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     4621 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     4681 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     4741 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     4801 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     4861 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     4921 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     4981 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg
     5041 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     5101 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     5161 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     5221 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     5281 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     5341 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     5401 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     5461 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     5521 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     5581 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     5641 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     5701 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     5761 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     5821 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     5881 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     5941 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     6001 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     6061 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     6121 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     6181 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt
     6241 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca
     6301 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     6361 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     6421 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     6481 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     6541 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     6601 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     6661 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     6721 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     6781 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     6841 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     6901 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     6961 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     7021 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     7081 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     7141 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     7201 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     7261 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     7321 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     7381 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
     7441 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     7501 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     7561 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     7621 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
     7681 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
     7741 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
     7801 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7861 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7921 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     7981 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     8041 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     8101 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8161 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8221 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8281 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8341 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8401 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8461 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8521 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8581 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8641 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8701 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8761 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8821 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8881 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8941 ggattttggt catgcatgat atatctccca atttgtgtag ggcttattat gcacgcttaa
     9001 aaataataaa agcagacttg acctgatagt ttggctgtga gcaattatgt gcttagtgca
     9061 tctaatcgct tgagttaacg ccggcgaagc ggcgtcggct tgaacgaatt tctagctaga
     9121 cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
     9181 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
     9241 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
     9301 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
     9361 tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
     9421 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
     9481 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
     9541 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
     9601 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
     9661 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
     9721 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg
     9781 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
     9841 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
     9901 accgcttccc ccatgatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     9961 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
    10021 cgaggcatag actgtacccc aaaaaaacat gtcataacaa gaagccatga aaaccgccac
    10081 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg acggcagtta
    10141 cgctacttgc attacagctt acgaaccgaa cgaggcttat gtccactggg ttcgtgcccg
    10201 aat
//
