LOCUS       sequence-100496-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of BFP, rtTA and 
            blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-BB
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctattcttgc gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta
       61 tttagcttct tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag
      121 atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc
      181 cacctaccag gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc
      241 acccccgaag gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct
      301 aaggtggtgg acttggacac cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc
      361 gtccgtggcc ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc
      421 atcgacaagg acggctggct gcacagcggc gacatcgcct actgggacga ggacgagcac
      481 ttcttcatcg tggaccggct gaagagcctg atcaaataca agggctacca ggtagcccca
      541 gccgaactgg agagcatcct gctgcaacac cccaacatct tcgacgccgg ggtcgccggc
      601 ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa
      661 accatgaccg agaaggagat cgtggactat gtggccagcc aggttacaac cgccaagaag
      721 ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg caagttggac
      781 gcccgcaaga tccgcgagat tctcattaag gccaagaagg gcggcaagat cgccgtgtaa
      841 ggcctgtcag gcc
//
