LOCUS       sequence-100501-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: GFP and
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-GH
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..78
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gagctgtaca agggcagtgg agctactaac ttcagcctgc tgaagcaggc tggtgacgtc
       61 gaggagaatc ctggccccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
      121 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
      181 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
      241 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
      301 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
      361 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
      421 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
      481 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
      541 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
      601 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
      661 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
      721 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
      781 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
      841 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaa
//
