LOCUS       sequence-100503-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of GFP, rtTA and 
            neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-GN
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat
       61 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga
      121 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga
      181 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct
      241 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt
      301 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact
      361 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt
      421 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga
      481 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat
      541 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc
      601 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc
      661 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt
      721 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt
      781 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc
      841 caagaagggc ggcaagatcg ccgtgtaagg cctgtcaggc ca
//
