LOCUS       sequence-100506-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of GFP, rtTA and 
            blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-GB
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt
       61 cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg
      121 cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg
      181 catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg
      241 ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga
      301 cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc
      361 catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga
      421 cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt
      481 ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga
      541 gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga
      601 cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga
      661 gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg
      721 tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat
      781 ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaag gcctgtcagg
      841 cc
//
