LOCUS       sequence-100507-       10502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for F-actin LifeAct-mCherry expressed under 
            MtBCP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMB-ACTLr
FEATURES             Location/Qualifiers
     source          1..10502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(648..1442)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(759..778)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1369..1388
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1476..1655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1773..1795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1792..1808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1792..1808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1828..1848
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3030..3054
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3057..3107
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             3108..3815
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3521..3540
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3817..3841)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4072..4092)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4100..4117)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4100..4116)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4109..4131)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4205..4229
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5530..6159
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6593..7660
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7726..7920
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8156..8178)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8264..8404
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8319..8338
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8419..8436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8590..9178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8670..8689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9422..10213)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcatg tatgataatt
     1741 cgagggtacc cggggatcct ctagagggcc cgagcggata acaatttcac acaggaaaca
     1801 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgga gagagggaga
     1861 tgtgttttta aggagctata gaaggataca ataccgcttc tttgattggt attctaactc
     1921 aatgcgttgc ttaagagttg gttaatctat ttgaaatgtt gcgattatag tagatcttgt
     1981 gctcccatct ttaatatatc ttttacactt actccttagt tatacaaaca ttaaacatca
     2041 taacttctaa ggtaattgat acgtgccaaa ttttctaaac tccattaaat ttggtttgaa
     2101 agaaatttca tgaaattcct ctagttcgtt ataaatatta gataattagg ttttgagtag
     2161 cattgtagct accatgacct atttccatta catttgtagg tttaaaagat caaagatgac
     2221 ttggggtatc aattaacgag tgagcatata tgcgagcaag gaaagagata agtcagagtg
     2281 tttaaggaaa gcaaggcaag aaaattcact tagggagcca aaacaaggtc aatactcgat
     2341 tacatctcta atttaagctt gagaacttga tatattcgag actcctcaag tatttgtact
     2401 ccaagtgtca atactcgatt acatctctaa ttttccaaat aaagaaactc gtaagaaaca
     2461 aacattattt atgtgtaaac tttctgcatg gagacaactc aattataacg aagcccggaa
     2521 gaggaactat atatactgtt tctatttccc tttgacacgg tccacactat attgcatact
     2581 tgtttactgc caaataaaaa caagtatgtc cacgtggtta aataaaaaca aaagaaaact
     2641 ttctttttgt tcaagtgata gaaagtttct ttacattaat ataaaataat ataagtgtat
     2701 tccaaaaagg taaaaaaaaa aataataagt tgtggaaatt caacctcgtc ttagtaaaaa
     2761 gccgaccaaa tccggtagcc gtttataata gcaaacaaat attctattat tgttgtgacg
     2821 ataaaatcaa tacacccaca catgacaact atatatagcc taagttgata tgcacaattc
     2881 atcaaatcaa atcaaaacaa ttacatttgc taacttgcta ttcctttgat tgagatttta
     2941 ttctaggaaa taaaattttg acactcattt ttttaaagtt aatttctaca tttagagaga
     3001 aatattttcc aaaatcaaat tttgatcaag caagtttgta caaaaaagca ggcttcatgg
     3061 gtgtcgcaga tttgatcaag aaattcgaaa gcatctcaaa ggaagaaatg gtgagcaagg
     3121 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     3181 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     3241 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     3301 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     3361 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     3421 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     3481 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     3541 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     3601 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     3661 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     3721 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     3781 gccactccac cggcggcatg gacgagctgt acaaggaccc agctttcttg tacaaagtgg
     3841 aataagcggc catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct
     3901 ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta
     3961 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa
     4021 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac gcaactttat
     4081 tatacatagt tgataattca ctggccgtcg ttttacaacg tcgtgactgg gaaaacgagc
     4141 tcaagcttag cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag
     4201 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
     4261 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     4321 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag
     4381 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     4441 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     4501 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
     4561 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
     4621 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
     4681 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
     4741 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
     4801 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     4861 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     4921 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
     4981 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     5041 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     5101 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
     5161 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
     5221 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
     5281 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
     5341 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
     5401 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     5461 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     5521 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     5581 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     5641 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     5701 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     5761 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     5821 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     5881 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     5941 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     6001 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     6061 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     6121 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     6181 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     6241 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     6301 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     6361 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     6421 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     6481 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc
     6541 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa
     6601 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     6661 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     6721 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     6781 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     6841 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     6901 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     6961 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     7021 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     7081 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     7141 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     7201 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     7261 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     7321 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     7381 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     7441 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     7501 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     7561 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     7621 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     7681 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
     7741 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     7801 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     7861 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     7921 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
     7981 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
     8041 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
     8101 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     8161 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     8221 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     8281 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     8341 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     8401 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8521 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8581 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8641 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8701 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8761 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8821 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8881 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8941 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9001 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9061 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9121 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9241 gattttggtc atgcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa
     9301 aataataaaa gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat
     9361 ctaatcgctt gagttaacgc cggcgaagcg gcgtcggctt gaacgaattt ctagctagac
     9421 attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact
     9481 gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa
     9541 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
     9601 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
     9661 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
     9721 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
     9781 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
     9841 ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
     9901 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
     9961 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
    10021 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
    10081 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
    10141 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
    10201 ccgcttcccc catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt
    10261 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
    10321 gaggcataga ctgtacccca aaaaaacatg tcataacaag aagccatgaa aaccgccact
    10381 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga cggcagttac
    10441 gctacttgca ttacagctta cgaaccgaac gaggcttatg tccactgggt tcgtgcccga
    10501 at
//
