LOCUS       sequence-100508-       10510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 10510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMB-ACTLg
FEATURES             Location/Qualifiers
     source          1..10510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(648..1442)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(759..778)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1369..1388
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1476..1655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1773..1795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1792..1808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1792..1808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1828..1848
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3030..3054
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3057..3107
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             join(3108..3110,3111..3113,3114..3824)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3108..3110,3111..3113,3114..3824)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3153..3174)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3414..3433)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3761..3782
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3826..3850)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4081..4101)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4109..4126)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4109..4125)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4118..4140)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4214..4238
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5538..6167
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6601..7668
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7734..7928
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8164..8186)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8272..8412
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8327..8346
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8427..8444)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8598..9186)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8678..8697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9430..10221)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcatg tatgataatt
     1741 cgagggtacc cggggatcct ctagagggcc cgagcggata acaatttcac acaggaaaca
     1801 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgga gagagggaga
     1861 tgtgttttta aggagctata gaaggataca ataccgcttc tttgattggt attctaactc
     1921 aatgcgttgc ttaagagttg gttaatctat ttgaaatgtt gcgattatag tagatcttgt
     1981 gctcccatct ttaatatatc ttttacactt actccttagt tatacaaaca ttaaacatca
     2041 taacttctaa ggtaattgat acgtgccaaa ttttctaaac tccattaaat ttggtttgaa
     2101 agaaatttca tgaaattcct ctagttcgtt ataaatatta gataattagg ttttgagtag
     2161 cattgtagct accatgacct atttccatta catttgtagg tttaaaagat caaagatgac
     2221 ttggggtatc aattaacgag tgagcatata tgcgagcaag gaaagagata agtcagagtg
     2281 tttaaggaaa gcaaggcaag aaaattcact tagggagcca aaacaaggtc aatactcgat
     2341 tacatctcta atttaagctt gagaacttga tatattcgag actcctcaag tatttgtact
     2401 ccaagtgtca atactcgatt acatctctaa ttttccaaat aaagaaactc gtaagaaaca
     2461 aacattattt atgtgtaaac tttctgcatg gagacaactc aattataacg aagcccggaa
     2521 gaggaactat atatactgtt tctatttccc tttgacacgg tccacactat attgcatact
     2581 tgtttactgc caaataaaaa caagtatgtc cacgtggtta aataaaaaca aaagaaaact
     2641 ttctttttgt tcaagtgata gaaagtttct ttacattaat ataaaataat ataagtgtat
     2701 tccaaaaagg taaaaaaaaa aataataagt tgtggaaatt caacctcgtc ttagtaaaaa
     2761 gccgaccaaa tccggtagcc gtttataata gcaaacaaat attctattat tgttgtgacg
     2821 ataaaatcaa tacacccaca catgacaact atatatagcc taagttgata tgcacaattc
     2881 atcaaatcaa atcaaaacaa ttacatttgc taacttgcta ttcctttgat tgagatttta
     2941 ttctaggaaa taaaattttg acactcattt ttttaaagtt aatttctaca tttagagaga
     3001 aatattttcc aaaatcaaat tttgatcaag caagtttgta caaaaaagca ggcttcatgg
     3061 gtgtcgcaga tttgatcaag aaattcgaaa gcatctcaaa ggaagaaatg gtgagcaagg
     3121 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3181 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3241 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3301 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3361 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3421 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3481 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3541 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3601 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3661 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3721 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3781 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggaccca gctttcttgt
     3841 acaaagtgga ataagcggcc atgctagagt ccgcaaaaat caccagtctc tctctacaaa
     3901 tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta
     3961 gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg
     4021 tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacg
     4081 caactttatt atacatagtt gataattcac tggccgtcgt tttacaacgt cgtgactggg
     4141 aaaacgagct caagcttagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     4201 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     4261 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     4321 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     4381 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     4441 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     4501 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     4561 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     4621 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     4681 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     4741 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     4801 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     4861 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     4921 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     4981 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     5041 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     5101 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     5161 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     5221 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     5281 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     5341 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     5401 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     5461 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     5521 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     5581 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     5641 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     5701 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     5761 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     5821 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     5881 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     5941 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     6001 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     6061 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     6121 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     6181 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     6241 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     6301 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     6361 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     6421 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     6481 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     6541 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac
     6601 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     6661 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     6721 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     6781 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     6841 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     6901 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     6961 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     7021 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     7081 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     7141 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     7201 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     7261 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     7321 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     7381 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     7441 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     7501 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     7561 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     7621 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     7681 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     7741 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     7801 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     7861 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     7921 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     7981 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     8041 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     8101 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     8161 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     8221 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     8281 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     8341 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     8401 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8461 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8521 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8581 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8641 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8701 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8761 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8821 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8881 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8941 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9001 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     9061 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9121 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9181 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9241 cgttaaggga ttttggtcat gcatgatata tctcccaatt tgtgtagggc ttattatgca
     9301 cgcttaaaaa taataaaagc agacttgacc tgatagtttg gctgtgagca attatgtgct
     9361 tagtgcatct aatcgcttga gttaacgccg gcgaagcggc gtcggcttga acgaatttct
     9421 agctagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
     9481 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
     9541 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
     9601 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
     9661 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
     9721 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     9781 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     9841 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
     9901 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
     9961 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
    10021 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
    10081 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
    10141 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
    10201 ggcgatcacc gcttccccca tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt
    10261 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
    10321 ggatgcccga ggcatagact gtaccccaaa aaaacatgtc ataacaagaa gccatgaaaa
    10381 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgacg
    10441 gcagttacgc tacttgcatt acagcttacg aaccgaacga ggcttatgtc cactgggttc
    10501 gtgcccgaat
//
