LOCUS       sequence-100511-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of RFP, rtTA and 
            neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-RN
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca
       61 cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg
      121 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct
      181 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga
      241 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct
      301 gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc
      361 tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga
      421 gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc
      481 cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc
      541 cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg
      601 taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa
      661 gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt
      721 ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt
      781 gtaaggcctg tcaggcc
//
