LOCUS       sequence-100522-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: RFP and
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-RH
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..135
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg catggacgag
       61 ctgtacaagg gcagtggagc tactaacttc agcctgctga agcaggctgg tgacgtcgag
      121 gagaatcctg gccccatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
      181 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
      241 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
      301 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
      361 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
      421 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
      481 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
      541 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
      601 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
      661 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
      721 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
      781 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
      841 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
      901 cttgcaggat
//
