LOCUS       sequence-100523-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: BFP and
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-BH
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..727
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             737..793
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tgtagaaacc tctgcacctg aggccaccat gagcgagctg attaaggaga acatgcacat
       61 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
      121 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
      181 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
      241 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
      301 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
      361 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
      421 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
      481 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
      541 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
      601 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
      661 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
      721 gctgaacggc agtggagcta ctaacttcag cctgctgaag caggctggtg acgtcgagga
      781 gaatcctggc cccatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
      841 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcga
//
