LOCUS       sequence-10053-e        8723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK
  TITLE     Oligomerized pool engineering (OPEN): an 'open-source' protocol for 
            making customized zinc-finger arrays.
  JOURNAL   Nat Protoc. 2009. 4(10):1471-501.
  PUBMED    19798082
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2009. 4(10):1471-501."
COMMENT     Sequence Label: pKJ1712
FEATURES             Location/Qualifiers
     source          1..8723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1392)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2858..3673)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2858..3673)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3581..3600
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3665..3684
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      3984..4070
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4162..4189
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     4267..4285
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(4381..4386,4387..4404,4405..4411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     4421..4443
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4440..4456
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4452..5114
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             5162..5953
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     complement(5961..5978)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5961..5977)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5970..5992)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(join(6833..8167,8168..8254))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(8255..8700)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 gcggtccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct
      961 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     1021 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     1081 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     1141 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     1201 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     1261 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     1321 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     1381 aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa
     1441 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca
     1501 tgtttgacag cttatcatcg ataagctagc tttaatgcgg tagtttatca cagttaaatt
     1561 gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca
     1621 ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggttgcacaa tcttctcgcg
     1681 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
     1741 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
     1801 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
     1861 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
     1921 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
     1981 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
     2041 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
     2101 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc
     2161 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
     2221 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
     2281 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
     2341 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     2401 gaaagcgggc agtgagcgca acgcaattaa tgtaagttag cgcgaattat cgtccattcc
     2461 gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta
     2521 tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct
     2581 tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtgggat
     2641 cagatctcgc aattgccgtt gatttgggga tctttgttgt aggtggacca gttggtgatt
     2701 ttgaactttt gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc
     2761 ttcaactcag caaaagttcg atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa
     2821 tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca
     2881 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     2941 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     3001 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     3061 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaga
     3121 gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     3181 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     3241 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     3301 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     3361 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     3421 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     3481 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     3541 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     3601 catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac gtttcccgtt
     3661 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     3721 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc
     3781 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca
     3841 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc cacctgaccc
     3901 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
     3961 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     4021 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
     4081 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
     4141 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
     4201 aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gacattaacc
     4261 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattctgtgg aagatcttga
     4321 agagcgcggc cgcgatgacc gtaggcgcgc cgctcttcta ttacattagg caccccgggc
     4381 tttacacttt atgcttccgg ctcgtatgtt gtgtcgaccg agcggataac aatttcacac
     4441 aggaaacagc tatgacagag cagaaagccc tagtaaagcg tattacaaat gaaaccaaga
     4501 ttcagattgc gatctcttta aagggtggtc ccctagcgat agagcactcg atcttcccag
     4561 aaaaagaggc agaagcagta gcagaacagg ccacacaatc gcaagtgatt aacgtccaca
     4621 caggtatagg gtttctggac catatgatac atgctctggc caagcattcc ggctggtcgc
     4681 taatcgttga gtgcattggt gacttacaca tagacgacca tcacaccact gaagactgcg
     4741 ggattgctct cggtcaagct tttaaagagg ccctaggggc cgtgcgtgga gtaaaaaggt
     4801 ttggatcagg atttgcgcct ttggatgagg cactttccag agcggtggta gatctttcga
     4861 acaggccgta cgcagttgtc gaacttggtt tgcaaaggga gaaagtagga gatctctctt
     4921 gcgagatgat cccgcatttt cttgaaagct ttgcagaggc tagcagaatt accctccacg
     4981 ttgattgtct gcgaggcaag aatgatcatc accgtagtga gagtgcgttc aaggctcttg
     5041 cggttgccat aagagaagcc acctcgccca atggtaccaa cgatgttccc tccaccaaag
     5101 gtgttcttat gtagtgacac cgattattta aagctgcagg gatcccacac aggaaacagc
     5161 tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     5221 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     5281 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     5341 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     5401 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     5461 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     5521 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     5581 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     5641 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     5701 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     5761 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     5821 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     5881 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     5941 agtcggcaaa taagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6001 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6061 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     6121 tttgcctggt ttccggcacc agaagcggtg ccggaagctg gctggagtgc gatcttcctg
     6181 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
     6241 acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
     6301 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc
     6361 gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg
     6421 gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg
     6481 gagaaaaccg cctcgcggtg atggtgctgc gttggagtga cggcagttat ctggaagatc
     6541 aggatatgtg gcggatgagc ggcattttcc gtgacgaatt tctgccattc atccgcttat
     6601 tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa
     6661 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     6721 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     6781 gccttgcgta taatatttgc ccatggccgg atcggcattt tcttttgcgt ttttatttgt
     6841 taactgttaa ttgtccttgt tcaaggatgc tgtctttgac aacagatgtt ttcttgcctt
     6901 tgatgttcag caggaagctt ggcgcaaacg ttgattgttt gtctgcgtag aatcctctgt
     6961 ttgtcatata gcttgtaatc acgacattgt ttcctttcgc ttgaggtaca gcgaagtgtg
     7021 agtaagtaaa ggttacatcg ttaggatcaa gatccatttt taacacaagg ccagttttgt
     7081 tcagcggctt gtatgggcca gttaaagaat tagaaacata accaagcatg taaatatcgt
     7141 tagacgtaat gccgtcaatc gtcatttttg atccgcggga gtcagtgaac aggtaccatt
     7201 tgccgttcat tttaaagacg ttcgcgcgtt caatttcatc tgttactgtg ttagatgcaa
     7261 tcagcggttt catcactttt ttcagtgtgt aatcatcgtt tagctcaatc ataccgagag
     7321 cgccgtttgc taactcagcc gtgcgttttt tatcgctttg cagaagtttt tgactttctt
     7381 gacggaagaa tgatgtgctt ttgccatagt atgctttgtt aaataaagat tcttcgcctt
     7441 ggtagccatc ttcagttcca gtgtttgctt caaatactaa gtatttgtgg cctttatctt
     7501 ctacgtagtg aggatctctc agcgtatggt tgtcgcctga gctgtagttg ccttcatcga
     7561 tgaactgctg tacattttga tacgtttttc cgtcaccgtc aaagattgat ttataatcct
     7621 ctacaccgtt gatgttcaaa gagctgtctg atgctgatac gttaacttgt gcagttgtca
     7681 gtgtttgttt gccgtaatgt ttaccggaga aatcagtgta gaataaacgg atttttccgt
     7741 cagatgtaaa tgtggctgaa cctgaccatt cttgtgtttg gtcttttagg atagaatcat
     7801 ttgcatcgaa tttgtcgctg tctttaaaga cgcggccagc gtttttccag ctgtcaatag
     7861 aagtttcgcc gactttttga tagaacatgt aaatcgatgt gtcatccgca tttttaggat
     7921 ctccggctaa tgcaaagacg atgtggtagc cgtgatagtt tgcgacagtg ccgtcagcgt
     7981 tttgtaatgg ccagctgtcc caaacgtcca ggccttttgc agaagagata tttttaattg
     8041 tggacgaatc gaactcagga acttgatatt tttcattttt ttgctgttca gggatttgca
     8101 gcatatcatg gcgtgtaata tgggaaatgc cgtatgtttc cttatatggc ttttggttcg
     8161 tttctttcgc aaacgcttga gttgcgcctc ctgccagcag tgcggtagta aaggttaata
     8221 ctgttgcttg ttttgcaaac tttttgatgt tcatcgttca tgtctccttt tttatgtact
     8281 gtgttagcgg tctgcttctt ccagccctcc tgtttgaaga tggcaagtta gttacgcaca
     8341 ataaaaaaag acctaaaata tgtaaggggt gacgccaaag tatacacttt gccctttaca
     8401 cattttaggt cttgcctgct ttatcagtaa caaacccgcg cgatttactt ttcgacctca
     8461 ttctattaga ctctcgtttg gattgcaact ggtctatttt cctcttttgt ttgatagaaa
     8521 atcataaaag gatttgcaga ctacgggcct aaagaactaa aaaatctatc tgtttctttt
     8581 cattctctgt attttttata gtttctgttg catgggcata aagttgcctt tttaatcaca
     8641 attcagaaaa tatcataata tctcatttca ctaaataata gtgaacggca ggtatatgtg
     8701 atgggttaaa aaggatctga tga
//
