LOCUS       sequence-100531-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: BFP and
            neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-BN
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..796
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             806..862
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tccatagaca tctgacctcg gcacttagca tcatcacagc aaactaactg tagcctttct
       61 ctctttccct gtagaaacct ctgcacctga ggccaccatg agcgagctga ttaaggagaa
      121 catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc
      181 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
      241 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
      301 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
      361 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
      421 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
      481 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
      541 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag
      601 ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa
      661 gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
      721 gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact
      781 ggggcacaag ctgaacggca gtggagctac taacttcagc ctgctgaagc aggctggtga
      841 cgtcgaggag aatcctggcc ccatgattga acaagatgga ttgcacgcag
//
