LOCUS       sequence-100534-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with constitutive bi-directional promoter, one side: 
            SfiI cloning site for GOI (contains filler DNA), other side: BFP and
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBbi-BP
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             126..824
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 ggttcctgcg ttgggattcc gtgtacaatc catagacatc tgacctcggc acttagcatc
       61 atcacagcaa actaactgta gcctttctct ctttccctgt agaaacctct gcacctgagg
      121 ccaccatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
      181 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
      241 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
      301 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
      361 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
      421 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
      481 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
      541 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
      601 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata
      661 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
      721 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
      781 ccagatactg cgacctccct agcaaactgg ggcacaagct gaacggcagt ggagctacta
      841 acttcagcct gctgaagcag gc
//
