LOCUS       sequence-100536-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of rtTA and 
            blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-Bla
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac
       61 cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg
      121 ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta
      181 agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc
      241 gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc
      301 agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg
      361 cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag
      421 cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag
      481 gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg
      541 gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc
      601 taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac
      661 gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg
      721 ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt
      781 acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg
      841 accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc
      901 aagatcgccg tgtaaggcct gtcaggccaa gcttccatc
//
