LOCUS       sequence-100540-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of rtTA and neomycin
            resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-Neo
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgctggtgcc cacactattt agcttcttcg ctaagagcac tctcatcgac aagtacgacc
       61 taagcaactt gcacgagatc gccagcggcg gggcgccgct cagcaaggag gtaggtgagg
      121 ccgtggccaa acgcttccac ctaccaggca tccgccaggg ctacggcctg acagaaacaa
      181 ccagcgccat tctgatcacc cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg
      241 tgcccttctt cgaggctaag gtggtggact tggacaccgg taagacactg ggtgtgaacc
      301 agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt aacaaccccg
      361 aggctacaaa cgctctcatc gacaaggacg gctggctgca cagcggcgac atcgcctact
      421 gggacgagga cgagcacttc ttcatcgtgg accggctgaa gagcctgatc aaatacaagg
      481 gctaccaggt agccccagcc gaactggaga gcatcctgct gcaacacccc aacatcttcg
      541 acgccggggt cgccggcctg cccgacgacg atgccggcga gctgcccgcc gcagtcgtcg
      601 tgctggaaca cggtaaaacc atgaccgaga aggagatcgt ggactatgtg gccagccagg
      661 ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg cctaaaggac
      721 tgaccggcaa gttggacgcc cgcaagatcc gcgagattct cattaaggcc aagaagggcg
      781 gcaagatcgc cgtgtaaggc ctgtcaggcc aagctt
//
