LOCUS       sequence-100543-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of RFP, rtTA and 
            blasticidin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-RB
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca gcttgcaaga
       61 ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac
      121 tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct
      181 cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg
      241 ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc
      301 tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacaccgg
      361 taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag
      421 cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca
      481 cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa
      541 gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct
      601 gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga
      661 gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt
      721 ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt
      781 ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct
      841 cattaaggcc aagaagggcg gcaagatcgc cgtgtaaggc ctgtcaggcc aagctt
//
