LOCUS       sequence-100547-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of rtTA and 
            hygromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-Hyg
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgcttcga ggaggagcta ttcttgcgca gcttgcaaga ctataagatt caatctgccc
       61 tgctggtgcc cacactattt agcttcttcg ctaagagcac tctcatcgac aagtacgacc
      121 taagcaactt gcacgagatc gccagcggcg gggcgccgct cagcaaggag gtaggtgagg
      181 ccgtggccaa acgcttccac ctaccaggca tccgccaggg ctacggcctg acagaaacaa
      241 ccagcgccat tctgatcacc cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg
      301 tgcccttctt cgaggctaag gtggtggact tggacaccgg taagacactg ggtgtgaacc
      361 agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt aacaaccccg
      421 aggctacaaa cgctctcatc gacaaggacg gctggctgca cagcggcgac atcgcctact
      481 gggacgagga cgagcacttc ttcatcgtgg accggctgaa gagcctgatc aaatacaagg
      541 gctaccaggt agccccagcc gaactggaga gcatcctgct gcaacacccc aacatcttcg
      601 acgccggggt cgccggcctg cccgacgacg atgccggcga gctgcccgcc gcagtcgtcg
      661 tgctggaaca cggtaaaacc atgaccgaga aggagatcgt ggactatgtg gccagccagg
      721 ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg cctaaaggac
      781 tgaccggcaa gttggacgcc cgcaagatcc gcgagattct cattaaggcc aagaagggcg
      841 gcaagatcgc cgtgtaaggc ctgtcaggcc a
//
