LOCUS       sequence-100551-         565 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 with single cysteine residue (M1C) for site-specific labelling
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 565)
  AUTHORS   O'Connell MR, Oakes BL, Sternberg SH, East-Seletsky A, Kaplan M, 
            Doudna JA
  TITLE     Programmable RNA recognition and cleavage by CRISPR/Cas9.
  JOURNAL   Nature. 2014 Sep 28. doi: 10.1038/nature13769.
  PUBMED    25274302
REFERENCE   2  (bases 1 to 565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Sep 28. doi: 10.1038/nature13769."
COMMENT     Sequence Label: 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S...
FEATURES             Location/Qualifiers
     source          1..565
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..44
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ataacaacaa cctcggnatc gaggaaaacc tgtacttcca atccaatgca tgcgataaga
       61 aatactcaat aggcttagct atcggcncaa atagcgtcng atgggcggtg atcactgatg
      121 aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca
      181 aaaaaaatct tatangggct cttttatttg acagtggaga gacagcggaa gcgactcgtc
      241 tcaaacggac agctcgtana aggtatacac gtcggangaa tcgtatttct tatctacagg
      301 agattttttc aaatganatg gcgaaagtag atgatanttt ctttcatcna cttgaagagt
      361 cttttttggt ggaagaagac aanaancatg aacgtcatcc tatttttgga aatatagtag
      421 atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggnag
      481 attctactga tnaagcggat ttgcgcttan tctatttggc cttancgcat atgattaagt
      541 ttcgtggtca ttttttgatt gaggg
//
