LOCUS       sequence-100552-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 with single cysteine residue (M1C) for site-specific labelling
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   O'Connell MR, Oakes BL, Sternberg SH, East-Seletsky A, Kaplan M, 
            Doudna JA
  TITLE     Programmable RNA recognition and cleavage by CRISPR/Cas9.
  JOURNAL   Nature. 2014 Sep 28. doi: 10.1038/nature13769.
  PUBMED    25274302
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Sep 28. doi: 10.1038/nature13769."
COMMENT     Sequence Label: 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S...
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(24..71)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     67..85
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ccggatatag ttcctccttt cancaaaaaa cccctcaaga cccgtttaga ggccccaagg
       61 ggttatgcta gttattgctc agcggtggca gcagccaact cagcttcctt tcgggctttg
      121 ttagcagccg gatcagcgcg cacgcgtggt accggccggc caccaggtgg cgcgccgcga
      181 tcgcggatcc gttatccact tccaatgtta ttagtcacct cctagctgac tcaaatcaat
      241 gcgtgtttca taaagaccag tgatggattg atggataaga gtggcatcta aaacttcttt
      301 tgtagacgta tatcgtttac gatcaattgt tgtatcaaaa tatttaaaag cagcgggagc
      361 tccaagattc gtcaacgtaa ataaatgaat aatattttct gcttgttcac gtattggttt
      421 gtctctatgt ttgttatatg cactaagaac tttatctaaa ttggcatctg ctaaaataac
      481 acgcttagaa aattcactga tttgctcaat aatctcatct aaataatgct tatgctgctc
      541 cacaaacaat tgtttttgtt cgttatcttc
//
