LOCUS       sequence-100554-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Contains the 
            miR-124-regulated 3′UTR of the Vamp3 gene with one seed site 
            mutated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: sgFFL_d3
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agatatgact atgtttactt tcttgttctt gttgtatatc actcctaaaa tagtatttta
       61 accagaaata accctttaag aagtttttat agaactatga ccaaagtttc tattttgcca
      121 aaagttaagt acaaataaat tgccccaata gatacattca aataagtgcc aaatgtaact
      181 caaggtattc tttaagaaaa atgttaatac tgtgagagtg agccttcaat atgtcggcag
      241 tccatcctgt gaaatgctat agagaaggct agtcattctg atttggtttc ttctttagag
      301 ctatggaggt gggctcatat cacacctgta gacaagatac ttagccagca gtttgtagac
      361 tgagctaggc tgtggtctct agtgcaatgt gagcagtagc cgtgcagcct caccaaggca
      421 tcagtctgca cacagttcca gaagactgtg tactgcttgt actcatctct gtcttatgct
      481 tgaggtaagc caccggtcac tcgtagagga tagctaacca gagatgcctg gtgctcttag
      541 aatgtagcca ttgctgtctg ggatagtttc attgtttata tgagactttc atcaaggctt
      601 ggttcaaaga caggagttca ggcatgggtg gaattgagga agtggtgaag ggaaagctgc
      661 ttgcctatgg cagtgccact agaatgtcct gaaatctgtg tttgtgtgta aagcagcatt
      721 actcaagatg tcatagaatc tggttctagt tttttgtttt tgttttgttt tgttttttga
      781 gacagggttt ctctgtatag cccttgctgt cctggaactc actttgtaga ccaggctggc
      841 tcgaactcaa aaatctgcct gcctctgcct cccaagtgca gggattaaag gcgtgtgcca
      901 ccaacgccca actggttcta gttttttatc tgttccccta acattactga aacaatacag
      961 atgttgaaga tatgccttgc cg
//
