LOCUS       sequence-100556-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Contains the 
            miR-124-regulated 3′UTR of the Vamp3 gene with one seed site 
            mutated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: sgFFL_d3
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cggccgctcg agtagccatg gcttcccgcc ggcggtggcg gcgcaggatg atggcacgct
       61 gcccatgtct tgtgcccagg agagcgggat ggaccgtcac cctgcagcct gtgcttctgc
      121 taggatcaat gtgtagataa gaatgggccc caccgatgaa actgaagccc gatattcagg
      181 atcttcttca agactttcta cttagaacct gccgtgttat cgagcttacc tattgttttt
      241 ttccaaatga ttatttttgt tagtttacat acagtttagt gcctagaaaa tgtgcctttg
      301 tttttcatat acactccaaa ctggaggtgt gacctaccct ccccacattt tgcacagtgc
      361 ctttacagat ttctggactg ggaaagagga cttgtgtagt tctgagtgct gaatggaggt
      421 ggatgttgtc actcaagatg acttgttgtg gattccagga agggggtttt gttcaaggca
      481 gctgtcccca catctgacat catcttcaaa accacatttt aaacttttgg gtaattagat
      541 tcccaacttg gaccttccaa gaagaacact actgcctaac agcaatgggt gacaatagca
      601 gggtattttg atagctagga aactgttctt taagattatt ctccgtttgt aagcactact
      661 gactcccgct gtatttaaga tgctggtgaa gagctcatgc tcttatgtta gatatgacta
      721 tgtttacttt cttgttcttg ttgtatatca ctcctaaaat agtattttaa ccagaaataa
      781 ccctttaaga agtttttata gaactatgac caaag
//
