LOCUS       sequence-100558-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Contains the 
            miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites 
            mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: sgFFL_d23
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gactatgttt actttcttgt tcttgttgta tatcactcct aaaatagtat tttaaccaga
       61 aataaccctt taagaagttt ttatagaact atgaccaaag tttctatttt gccaaaagtt
      121 aagtacaaat aaattgcccc aatagataca ttcaaataag tgccaaatgt aactcaaggt
      181 attctttaag aaaaatgtta atactgtgag agtgagcctt caatatgtcg gcagtccatc
      241 ctgtgaaatg ctatagagaa ggctagtcat tctgatttgg tttcttcttt agagctatgg
      301 aggtgggctc atatcacacc tgtagacaag atacttagcc agcagtttgt agactgagct
      361 aggctgtggt ctctagtgca atgtgagcag tagccgtgca gcctcaccaa ggcatcagtc
      421 tgcacacagt tccagaagac tgtgtactgc ttgtactcat ctctgtctta tgcttgaggt
      481 aagccaccgg tcactcgtag aggatagcta accagagatg cctggtgctc ttagaatgta
      541 gccattgctg tctgggatag tttcattgtt tatatgagac tttcatcaag gcttggttca
      601 aagacaggag ttcaggcatg ggtggaattg aggaagtggt gaagggaaag ctgcttgcct
      661 atggcagtgc cactagaatg tcctgaaatc tgtgtttgtg tgtaaagcag cattactcaa
      721 gatgtcatag aatctggttc tagttttttg tttttgtttt gttttgtttt ttgagacagg
      781 gtttctctgt atagcccttg ctgtcctgga actcactttg tagaccaggc tggctcgaac
      841 tcaaaaatct gcctgcctct gcctcccaag tgcagggatt aaaggcgtgt gccaccaacg
      901 cccaactggt tctagttttt tatctgttcc cctaacatta ctgaaacaat acagatgttg
      961 aagatatgcc ttgccg
//
