LOCUS       sequence-100560-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Contains the 
            miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites 
            mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: sgFFL_d23
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cggccgctcg agtagccatg gcttcccgcc ggcggtggcg gcgcaggatg atggcacgct
       61 gcccatgtct tgtgcccagg agagcgggat ggaccgtcac cctgcagcct gtgcttctgc
      121 taggatcaat gtgtagataa gaatgggccc caccgatgaa actgaagccc gatattcagg
      181 atcttcttca agactttcta cttagaacct gccgtgttat cgagcttacc tattgttttt
      241 ttccaaatga ttatttttgt tagtttacat acagtttagt gcctagaaaa tgtgcctttg
      301 tttttcatat acactccaaa ctggaggtgt gacctaccct ccccacattt tgcacataca
      361 gatttctgga ctgggaaaga ggacttgtgt agttctgagt gctgaatgga ggtggatgtt
      421 gtcactcaag atgacttgtt gtggattcca ggaagggggt tttgttcaag gcagctgtcc
      481 ccacatctga catcatcttc aaaaccacat tttaaacttt tgggtaatta gattcccaac
      541 ttggaccttc caagaagaac actactgcct aacagcaatg ggtgacaata gcagggtatt
      601 ttgatagcta ggacaatagc agggtatttt gatagctagg aaactgttct ttaagattat
      661 tctccgtttg taagcactac tgactcccgc tgtatttaag atgctggtga agagctcatg
      721 ctcttatgtt agatatgact atgtttactt tcttgttctt gttgtatatc actcctaaaa
      781 tagtatttta accagaaata accctttaag aagtttttat agaactatga ccaaag
//
