LOCUS       sequence-100566-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Does not contain 
            the target containing Vamp3 3′UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: OpenLoopControl
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(426..437)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 agtactgctc agagctacaa ctctaggagt agggactcca agcctagagc tccaagagag
       61 ggtgaagggc agggagaaaa ttatagtaat agttgcaatg agtcacttgc ttctagatca
      121 agatcagaga ctctgctctc cgtgttcaca gcggaccttg atttaatgtc atacaattaa
      181 ggcacgcggt gaatgccaag agcggagcct acggctgcac ttgaaggaca tccgagagaa
      241 gttaggaagg gtggggagaa acaattctag aatgaaccca tcctgtgcga cacaaggacc
      301 cctcgcattg attcaaaatc tttagaatac aagacagtca aatgtttttc agcgagaatc
      361 tgggaaatcc gcatgcatac tcccaagggg aatattagag gtttcattga cagctgtcag
      421 gagagctgta gttcccattg gtgcaaacgg tctaagagat ggcactcaga gagtgctggc
      481 cttttccaaa tactagtcat atgcggccgc agcttgaggt gtgacaggct tgagatctgg
      541 ccatacactt gagtgacaat gacatccact ttgcctttct ctccacagct gcccggcgcc
      601 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      661 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtct
      721 gaccttgata tccagcacag tggcggccgc tcgagtagcc atggcttccc gccggcggtg
      781 gcggcgcagg atgatggcac gctgcccatg tcttgtgccc aggagagcgg gatggaccgt
      841 caccctgcag cctgtgcttc tgctaggatc aatgtgtagt aatgagttta aggcgcgcgg
//
