LOCUS       sequence-100568-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Contains the 
            miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: sgFFL_3x_siRNA
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(219..230)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cctacggctg cacttgaagg acatccgaga gaagttagga agggtgggga gaaacaattc
       61 tagaatgaac ccatcctgtg cgacacaagg acccctcgca ttgattcaaa atctttagaa
      121 tacaagacag tcaaatgttt ttcagcgaga atctgggaaa tccgcatgca tactcccaag
      181 gggaatatta gaggtttcat tgacagctgt caggagagct gtagttccca ttggtgcaaa
      241 cggtctaaga gatggcactc agagagtgct ggccttttcc aaatactagt catatgcggc
      301 cgcagcttga ggtgtgacag gcttgagatc tggccataca cttgagtgac aatgacatcc
      361 actttgcctt tctctccaca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
      421 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
      481 tccaccggcg gcatggacga gctgtacaag tctgaccttg atatccagca cagtggcggc
      541 cgctcgagta gccatggctt cccgccggcg gtggcggcgc aggatgatgg cacgctgccc
      601 atgtcttgtg cccaggagag cgggatggac cgtcaccctg cagcctgtgc ttctgctagg
      661 atcaatgtgt agtaatgagt ttaaggcgcg cggctctaga gcaccgatga aactgaagcc
      721 cgatattcag gatctttctg accgattcgt gcttcggcat tcaccgcgtg ccttacaaca
      781 gaggacataa gccctacgcc catgatctac tgacgggcat tcaccgcgtg ccttagtaat
      841 gggacagtat ccgcggcaag tcctagtgca atggggcatt caccgcgtgc cttagtagta
      901 gaggcgacgc gggtgcggtc atcactaata aggataggag gtggatgttg tcactcaaga
      961 tga
//
