LOCUS       sequence-100569-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Contains the 
            miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: sgFFL_3x_siRNA
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(778..789)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
       61 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcaggt gagtactccc
      121 tctcaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt
      181 tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa
      241 agacaatctt tttgttgtca agctgtttga ctcatgcacc cgctactctt caaccttgca
      301 gactaattaa tggcaactct gacagatgtg gcgggaggaa agctgaggtg ggagtactgc
      361 tcagagctac aactctagga gtagggactc caagcctaga gctccaagag agggtgaagg
      421 gcagggagaa aattatagta atagttgcaa tgagtcactt gcttctagat caagatcaga
      481 gactctgctc tccgtgttca cagcggacct tgatttaatg tcatacaatt aaggcacgcg
      541 gtgaatgcca agagcggagc ctacggctgc acttgaagga catccgagag aagttaggaa
      601 gggtggggag aaacaattct agaatgaacc catcctgtgc gacacaagga cccctcgcat
      661 tgattcaaaa tctttagaat acaagacagt caaatgtttt tcagcgagaa tctgggaaat
      721 ccgcatgcat actcccaagg ggaatattag aggtttcatt gacagctgtc aggagagctg
      781 tagttcccat tggtgcaaac gg
//
