LOCUS       sequence-100572-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent FPX sensor for PIP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: pPHT-PIP2
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(230..248)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     498..517
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaccatgg tgagcaagag cgaggaggtc
      121 atcaaagagt tcatgcgctt caaggtgcgc ttggagggct ccatgaacgg ccacgagttc
      181 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      241 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gatcatgtac
      301 ggctccaagg cgtacgtgaa gcaccccgcc gacgtccccg attacatgaa gctgtccttc
      361 cccgagggct tcaagtggga gcgcgtgatg cacttcgagg acggcggtct ggtgaccgtg
      421 actcaggaca cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
      481 aacttccccc ccgacggccc cgtaatgcag aagaagacct tgggctggga ttatgccacc
      541 gagcgcctgt accccgaaga cggcgtgctg aagggcgagc ttctggggcg cctgaagctg
      601 aaggacggcg gcctcaacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
      661 caactgcccg gctactactt cgtggacacc aagctgggca tcacctccca caacgaggac
      721 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctggg catggacga
//
