LOCUS       sequence-100575-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent FPX sensor for PIP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: pPHT-PIP2
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..81
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    615..671
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(746..769)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
       61 aactagaagg cacagtcgag ggcgcgctac tggatgttga gctccttcag gaagttctgc
      121 agctccttga agctcatctt gttgtccttg tttttgtcag cttttcgcaa gcaggagtga
      181 atccagtgct gtagcttctg acgctggtcc atggagcctg agtggtggat gatcttgtgc
      241 agccccagca cccagtgctg ggcatcagct ggcgatgggg cgatgaggtc tagtgtattg
      301 cgctggtcct tgaagacaat ggagaagcag cggtcctcgg gcacatcacg ggcgaacttc
      361 tccagaccct ccgtgcggtg ccccattcgc acctcctgaa tgtcctcgat ggagaacagc
      421 tgggactccg gggtccgcat gaccttgcgg gactcctgcc agatggtctt gcagtcctcc
      481 tgcaacttgt agaagcgctc tctcctccat gagctggact tcaccttcag gagctggctg
      541 cccttcagca gcgcctgtag atcctcatca tcctgtaggc cgtgcagggt caggaagtcc
      601 cggcccgagt ccatggatcc cgggcccgcg gtaccgtcga ctgcagaatt cgaagcttga
      661 gctcgagatc tcttgtaccg ctcgtccatg cccagacggt ggcggccctc ggagcgcccg
      721 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggtgtccaca
      781 cagtaatcgc cgggcagttg cacgggcttc ttggccatgt agatggtctc gaactcca
//
