LOCUS       sequence-100576-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic autoregulatory gene circuit made by inserting an intron 
            containing the mouse mir-124–3 gene into mCherry. Contains the 
            miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
  TITLE     MicroRNA-based single-gene circuits buffer protein synthesis rates 
            against perturbations.
  JOURNAL   ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. 
            Epub 2014 Jan 30.
  PUBMED    24847681
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001867";
            journalName: "ACS Synth Biol"; date: "2014-05-16- 16"; volume: "3"; 
            issue: "5"; pages: "324-31"
COMMENT     Sequence Label: sgFFL_7x_Bulge
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(170..181)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 agaaacaatt ctagaatgaa cccatcctgt gcgacacaag gacccctcgc attgattcaa
       61 aatctttaga atacaagaca gtcaaatgtt tttcagcgag aatctgggaa atccgcatgc
      121 atactcccaa ggggaatatt agaggtttca ttgacagctg tcaggagagc tgtagttccc
      181 attggtgcaa acggtctaag agatggcact cagagagtgc tggccttttc caaatactag
      241 tcatatgcgg ccgcagcttg aggtgtgaca ggcttgagat ctggccatac acttgagtga
      301 caatgacatc cactttgcct ttctctccac agctgcccgg cgcctacaac gtcaacatca
      361 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
      421 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtctgacctt gatatccagc
      481 acagtggcgg ccgctcgagt agccatggct tcccgccggc ggtggcggcg caggatgatg
      541 gcacgctgcc catgtcttgt gcccaggaga gcgggatgga ccgtcaccct gcagcctgtg
      601 cttctgctag gatcaatgtg tagtaatgag tttaaggcgc gcggctctag agcaccgatg
      661 aaactgaagc ccgatattca ggatcttaga gacgcctgcc acaacagcag taggcattca
      721 tttgtgcctt actgggcatt caaaagtgcc ttatgtggca ttcaaatgtg ccttatctgg
      781 cattcatatg tgccttacat ggcattcaat tgtgccttag cgggcattca agagtgcctt
      841 acaggcattc aatcgtgcct tacgtttgga gttgaaagct aactaatgga ggtggatgtt
      901 gtcactcaag atgac
//
