LOCUS       sequence-100577-         431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIalpha R C-D
FEATURES             Location/Qualifiers
     source          1..431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(102..407)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(204..223)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 caatatatcc tgctcaaaca ctgatagttt aaaccatagg tctcaccttt acgaagtctt
       61 cgtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc
      121 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
      181 ataacggaaa ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg
      241 atatgcacca ccgggtaaag ttcacggggg actttatctg acagcagacg tgcactggcc
      301 agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac
      361 agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct
      421 gttctcgtca g
//
