LOCUS       sequence-100582-       11498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for plastids MtRubisco1sp-mCherry expressed 
            under AtUBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 11498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMU-PLAr
FEATURES             Location/Qualifiers
     source          1..11498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..263
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(356..608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(648..1442)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(759..778)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1369..1388
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1476..1655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1773..1795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1792..1808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1792..1808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1828..1848
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3835..3859
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4102..4812
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4515..4534
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4814..4838)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5069..5089)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5097..5114)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5097..5113)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5106..5128)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5202..5226
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6526..7155
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7589..8656
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8722..8916
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9152..9174)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9260..9400
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9315..9334
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9415..9432)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9586..10174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9666..9685)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10418..11209)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcatg tatgataatt
     1741 cgagggtacc cggggatcct ctagagggcc cgagcggata acaatttcac acaggaaaca
     1801 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgag tctagctcaa
     1861 cagagctttt aacccaaatt ggtacaatag aatacaactt tagatcataa ttctcaaaag
     1921 aaagagattc cttagctatt ctatctgcca ctccatttcc ttctcggctt gtatgcacaa
     1981 gcataaaatc ctcaaacttg ctaagtagat actttatgtc ttggataatt ggattgagac
     2041 ttgacaagca taactttcat gtaaccaaag acacaagttg ctgagaatcc acctcaaaaa
     2101 tgatcttcct ataattgaat cgggataatg acagcacagc ccatctaaga gcctccactt
     2161 ctacttccag cacgcttctt acttttacca cagctcttgc acctaaccat aacaccttcc
     2221 ctgtatgatc gcgaagcacc caccctaagc cacattttaa tccttctgtt ggccatgccc
     2281 catcaaagtt gcacttaacc caagattgtg gtggagcttc ccatgtttct cgtctgtccc
     2341 gacggtgttg tggttggtgc tttccttaca ttctgagcct ctttccttct aatccactca
     2401 tctgcatctt cttgtgtcct tactaatacc tcattggttc caaattccct ccctttaagc
     2461 accagctcgt ttctgttctt ccacagcctc ccaagtatcc aagggactaa agcctccaca
     2521 ttcttcagat caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact
     2581 gatgatctag gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg
     2641 tgtatcattc ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca
     2701 cgagtgttaa atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca
     2761 agaataaatc gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga
     2821 tacaaaagtc attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa
     2881 aaattaaaag aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa
     2941 gaaattcact gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag
     3001 gaaaagaaat aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga
     3061 cccacggttc aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata
     3121 atgctaaaaa aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga
     3181 ccgttagaaa ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc
     3241 ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat
     3301 aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta
     3361 ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct
     3421 tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa
     3481 tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct
     3541 gtgttcgtta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat
     3601 gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt
     3661 agatatcatc ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat
     3721 ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt
     3781 ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acaggcaagt
     3841 ttgtacaaaa aagcaggctt catggctttg atctcctccg ccgctgtcac caccgttaac
     3901 cgcgtctccg ctaacttggt tgctccattc accggtctca aatcctcagc tggtttccca
     3961 gtgaccaaga agaccaacaa tgacattacc tccatcacaa gcaatggtgg aagagtgaac
     4021 tgtatgcagg tttggccacc aattggcaag aagaagtttg aaacactttc ataccttcca
     4081 ccccttactg aggatcaatt gatggtgagc aagggcgagg aggataacat ggccatcatc
     4141 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     4201 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     4261 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     4321 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     4381 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     4441 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     4501 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     4561 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     4621 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     4681 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     4741 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     4801 ctgtacaagt aagacccagc tttcttgtac aaagtggaat aagcggccat gctagagtcc
     4861 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
     4921 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     4981 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     5041 taattcctaa aaccaaaatc cagtgacgca actttattat acatagttga taattcactg
     5101 gccgtcgttt tacaacgtcg tgactgggaa aacgagctca agcttagctt gagcttggat
     5161 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
     5221 gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag
     5281 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
     5341 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     5401 cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     5461 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     5521 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
     5581 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
     5641 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
     5701 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
     5761 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
     5821 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
     5881 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
     5941 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
     6001 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
     6061 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
     6121 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
     6181 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
     6241 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
     6301 gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg
     6361 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     6421 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     6481 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     6541 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     6601 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     6661 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     6721 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     6781 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     6841 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     6901 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     6961 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     7021 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     7081 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     7141 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     7201 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     7261 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     7321 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     7381 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     7441 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     7501 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
     7561 acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg
     7621 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     7681 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     7741 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     7801 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     7861 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     7921 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     7981 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     8041 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     8101 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     8161 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     8221 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     8281 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     8341 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     8401 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     8461 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     8521 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     8581 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     8641 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     8701 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     8761 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     8821 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     8881 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     8941 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     9001 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     9061 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     9121 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9181 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9241 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     9301 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     9361 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     9421 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9481 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9541 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9601 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9661 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9721 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9781 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9841 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9901 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9961 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10021 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10081 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10141 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10201 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc atgatatatc
    10261 tcccaatttg tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg
    10321 atagtttggc tgtgagcaat tatgtgctta gtgcatctaa tcgcttgagt taacgccggc
    10381 gaagcggcgt cggcttgaac gaatttctag ctagacatta tttgccgact accttggtga
    10441 tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat
    10501 cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg
    10561 gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg
    10621 cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg
    10681 gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa
    10741 ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt
    10801 ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa
    10861 tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa
    10921 tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag
    10981 gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc
    11041 ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg
    11101 cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa
    11161 ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttcccccatg atgtttaact
    11221 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc
    11281 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa
    11341 aacatgtcat aacaagaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
    11401 gtcaaggttc tggaccagtt gcgtgacggc agttacgcta cttgcattac agcttacgaa
    11461 ccgaacgagg cttatgtcca ctgggttcgt gcccgaat
//
