LOCUS       sequence-100583-         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. Both 
            fragments are driven by PBAD. The C-terminal fragment has the point 
            mutation R756S. Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-R756S
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag gttactactg
       61 gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc ctgagcgcat
      121 caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc cactggagaa
      181 cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct ttgagtacgc
      241 tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt ttgacgggtc
      301 ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg gtcgcgcggt
      361 taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta agaaagtcaa
      421 cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta ccgtgaccga
      481 tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac tggctggtca
      541 atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga cgctggctta
      601 cgggtccaaa gagttcggct tccgtcaa
//
