LOCUS       sequence-100587-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            R756S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-R756S
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg ccgtatcagc
       61 cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat ctggttccct
      121 tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc gcaaggtaac
      181 gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa ggaaggttac
      241 tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc gttccctgag
      301 cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa gtctccactg
      361 gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt ctgctttgag
      421 tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct ggcgtttgac
      481 gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc
      541 gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt tgctaagaaa
      601 gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt agttaccgtg
      661 accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa ggcactggct
      721 ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt catgacgctg
      781 gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac cattcagcca
      841 gc
//
