LOCUS       sequence-100596-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Expression Vector for Cas9 H840A nickase and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, 
            Karlin-Neumann GA, Conklin BR
  TITLE     Systematic quantification of HDR and NHEJ reveals effects of locus, 
            nuclease, and cell type on genome-editing.
  JOURNAL   Sci Rep. 2016 Mar 31;6:23549. doi: 10.1038/srep23549.
  PUBMED    27030102
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-03-31"; volume: "6:23549. doi"; pages: " 
            10.1038/srep23549"
COMMENT     Sequence Label: pXCas9H840A
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
       61 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
      121 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
      181 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
      241 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
      301 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
      361 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
      421 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
      481 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
      541 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
      601 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
      661 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggacgctat
      721 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
      781 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
      841 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
      901 caaggccgag agaggcggcc tgagcgaa
//
