LOCUS       sequence-100597-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI dy Esp3I-ccdB C-D
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(167..172,173..190,191..197)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             251..910
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(317..336)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttcgtctctc acctgagacc aaagctgaac gagaaacgta aaatgatata aatatcaata
       61 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
      121 agtcactatg gcggcccctc gaggggccgc attaggcacc ccaggcttta cactttatgc
      181 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
      241 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      301 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      361 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      421 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
      481 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      541 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      601 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      661 gtttattgag aatatgtttt tcgtgtcagc caatccctgg gtgagtttca ccagttttga
      721 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
      781 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
      841 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
      901 cggggcgtaa tcta
//
