LOCUS       sequence-100598-        1244 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Expression Vector for FokI-dCas9 and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, 
            Karlin-Neumann GA, Conklin BR
  TITLE     Systematic quantification of HDR and NHEJ reveals effects of locus, 
            nuclease, and cell type on genome-editing.
  JOURNAL   Sci Rep. 2016 Mar 31;6:23549. doi: 10.1038/srep23549.
  PUBMED    27030102
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-03-31"; volume: "6:23549. doi"; pages: " 
            10.1038/srep23549"
COMMENT     Sequence Label: pXFokI-dCas9
FEATURES             Location/Qualifiers
     source          1..1244
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..82
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             89..676
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ccncngtttc tgggncactg cctgaatcac tttttttcag gttggaccgg tgccaccatg
       61 tcctaagaag aagcggaagg tgagcagcca acttgtgaag tctgaactcg aggagaaaaa
      121 atcagagttg agacacaagt tgaagtacgt gccacacgaa tacatcgagc ttatcgagat
      181 cgccagaaac agtacccagg ataggatcct tgagatgaaa gtcatggagt tctttatgaa
      241 ggtctacggt tatagaggaa agcaccttgg cggtagcaga aagcccgatg gcgccatcta
      301 tactgtcgga tctcctatcg attatggggt gatcgtggat accaaagctt actcaggcgg
      361 gtacaacttg cccataggac aagccgacga gatgcagcgg tatgtcgaag agaaccagac
      421 gcgcaacaag cacatcaacc ccaatgaatg gtggaaagtg tacccaagta gtgtgactga
      481 gttcaagttc ctgtttgtct ccggccactt taagggcaat tataaagctc agctcactag
      541 actcaatcac atcacaaact gcaacggagc tgtgttgtca gtggaggagc tcctgattgg
      601 aggcgagatg atcaaagccg gcacccttac actggaggag gtgcggcgga agttcaacaa
      661 tggagagatc aacttcggtg gcggtggatc cgataaaaag tattctattg gtttagccat
      721 cggcactaat tccgttggat gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa
      781 atttaaggtg ttggggaaca cagaccgtca ttcgattaaa aagaatctta tcggtgccct
      841 cctattcgat agtggcgaaa ccggcagagg cgactcgcct gaaacgaacc gctcggagaa
      901 ggtatacacg tcgcaagaac cgaatatgtt ncttacanga anttttanca tganatggcc
      961 aagttgacga ttctttcttt cnccgnttgn aaaatccctt ccntgntcaa aaagacanaa
     1021 acatgaacgg cnccccntnt ttggaaactt atagatnaag tggcctntca tgnaanntnc
     1081 ccacgatttn tccctccnaa aaanntngtt gnnncactga nnangcggan cngaggttan
     1141 ncacttggcn ttgncctntn aaaagtccnn gggccttttt cntttanggg tantaatcgg
     1201 naannngaan ggcnnaannt tcncccttng nnancctnac cntt
//
