LOCUS       sequence-100608-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-tagged PKA catalytic subunit targeted to the extranuclear 
            cytoplasm by addition of a nuclear export signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Bok J, Zha XM, Cho YS, Green SH
  TITLE     An extranuclear locus of cAMP-dependent protein kinase action is 
            necessary and sufficient for promotion of spiral ganglion neuronal 
            survival by cAMP.
  JOURNAL   J Neurosci. 2003 Feb 1;23(3):777-87.
  PUBMED    12574406
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2003-02-1"; volume: "23(3)"; pages: "777-87"
COMMENT     Sequence Label: GPKAnes
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..133
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             248..268
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             290..310
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    440..561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(477..496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     531..550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(705..726)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
       61 gtccggactc agatctcgag agatggacta caaggacgac gatgacaagg actacaagga
      121 cgacgatgac aagaattcta ctgatgtgga aactacgtat gcagatttta ttgcctcagg
      181 aagaacaggt agaagaaatg caatacatga tatcttggtt tcctctgcaa gtggcaacag
      241 caatgaacct aagaagaaac gcaaggtcgg taccggagga ggtgaagcac ccaagaaaaa
      301 gcgaaaagta ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
      361 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
      421 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      481 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      541 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
      601 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
      661 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
      721 gtccactatt aaagaacgtg gactcccaac gtcaaagggc gaaaaaccgt ctatcagggc
      781 gatgggccca ctacgtgaac catcacccta atcaagtttt ttgggggtcg agggggccgt
      841 aaagcactaa atcnggaacc ctnaaagggn gc
//
