LOCUS       sequence-100627-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible expression of SARS PLpro protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Lee H, Cao S, Hevener KE, Truong L, Gatuz JL, Patel K, Ghosh AK, 
            Johnson ME
  TITLE     Synergistic inhibitor binding to the papain-like protease of human 
            SARS coronavirus: mechanistic and inhibitor design implications.
  JOURNAL   ChemMedChem. 2013 Aug;8(8):1361-72. doi: 10.1002/cmdc.201300134. 
            Epub 2013 Jun 20.
  PUBMED    23788528
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cmdc.201300134"; journalName: "ChemMedChem"; date: 
            "2013-08"; volume: "8"; issue: "8"; pages: "1361-72"
COMMENT     Sequence Label: pET15b SARS-CoV PLpro
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             949..972
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             973..990
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggaagtaa aaaccattaa agtgtttacc actgtggata acacgaatct gcacacccag
       61 ctggtggaca tgagcatgac gtatggtcag cagttcggcc cgacttacct ggacggtgcg
      121 gacgtaacca aaattaaacc gcacgtcaac cacgaaggca aaaccttttt cgttctgccg
      181 agcgacgata ccctgcgctc tgaagcgttc gaatactacc acaccctgga cgaatctttc
      241 ctgggtcgtt acatgtctgc gctgaaccac actaaaaaat ggaaattccc gcaggttggc
      301 ggtctgacca gcattaaatg ggcagacaac aactgctacc tgtccagcgt tctgctggcc
      361 ctgcaacagc tggaggttaa gttcaacgcg ccggctctgc aggaagcgta ttaccgtgca
      421 cgcgcgggtg atgcggcaaa cttctgtgct ctgatcctgg cttattccaa caagaccgtg
      481 ggcgaactgg gcgatgttcg tgaaaccatg acccacctgc tgcaacatgc taatctggaa
      541 tccgctaaac gcgtactgaa cgtggtctgt aaacattgcg gccagaaaac taccaccctg
      601 accggcgttg aagcagtgat gtatatgggt actctgtcct atgacaacct gaaaacgggc
      661 gtttctatcc cgtgcgtttg tggtcgtgat gcaactcagt acctggtaca gcaggaatcc
      721 tccttcgtta tgatgtctgc tccacctgcc gaatataaac tgcagcaagg cacctttctg
      781 tgcgcaaacg agtatactgg taactaccag tgcggtcact acactcacat cacggccaag
      841 gagaccctgt accgtatcga tggtgcccac ctgaccaaaa tgtctgaata caaaggcccg
      901 gtaacggacg tcttctacaa agagaccagc tacaccacta ctatcaagct ggaagttctg
      961 tttcagggtc cacaccatca ccaccatcat taa
//
