LOCUS       sequence-100632-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PTDH mutant Opt12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   McLachlan MJ, Johannes TW, Zhao H
  TITLE     Further improvement of phosphite dehydrogenase thermostability by 
            saturation mutagenesis.
  JOURNAL   Biotechnol Bioeng. 2008 Feb 1;99(2):268-74.
  PUBMED    17615560
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng."; date: "2008-02-1"; volume: "99(2)"; pages: "268-74"
COMMENT     Sequence Label: pET15b-Opt12
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctgccga aactcgttat aactcaccga gtacacgaag agatcctgca actgctggcg
       61 ccacattgcg agctgataac caaccagacc gacagcacgc tgacgcgcga ggaaattctg
      121 cgccgctgtc gcgatgctca ggcgatgatg gcgttcatgc ccgatcgggt cgatgcagac
      181 tttcttcaag cctgccctga gctgcgtgta atcggctgcg cgctcaaggg cttcgacaat
      241 ttcgatgtgg acgcctgtac tgcccgcggg gtctggctga ccttcgtgcc tgatctgttg
      301 acggtcccga ctgccgagct ggcgatcgga ctggcggtgg ggctggggcg gcatctgcgg
      361 gcagcagatg cgttcgtccg ctctggcaag ttcaagggct ggcaaccaca tttttacggc
      421 acggggctgg ataacgctac ggtcggcttc cttggcatgg gcgccatcgg actggccatg
      481 gctgatcgct tgcagggatg gggcgcgacc ctgcagtacc acgcggcgaa ggctctggat
      541 acacaaaccg agcaacggct cggcctgcgc caggtggcgt gcagcgaact cttcgccagc
      601 tcggacttca tcctgctggc gcttcccttg aatgccgata ccctgcatct ggtcaacgcc
      661 gagctgcttg ccctcgtacg gccgggcgct ctgcttgtaa acccctgtcg tggctcggta
      721 gtggatgaag ccgccgtgct cgcggcgctt gagcgaggcc agctcggcgg gtatgcggcg
      781 gatgtattcg aaatggaaga ctgggctcgc gcggaccggc cgctgtgcat cgatcctgcg
      841 ctgctcgcgc atccgaatac gctgttcact ccgcacatag ggtcggcagt gcgcgcggtg
      901 cgcctggaga ttgaacgttg tgcagcgcag aacatcctcc aggcattggc aggtgagcgc
      961 ccaatcaacg ctgtgaaccg tctgcccaag gccaatcctg ccgcagactg ataa
//
