LOCUS       sequence-100634-        4407 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E
  TITLE     A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive 
            Bacterial Immunity.
  JOURNAL   Science. 2012 Aug 17;337(6096):816-21. doi: 10.1126/science.1225829.
            Epub 2012 Jun 28.
  PUBMED    22745249
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1225829"; journalName: "Science"; date: 
            "2012-08-17- 17"; volume: "337"; issue: "6096"; pages: "816-21"
COMMENT     Sequence Label: pMJ823
FEATURES             Location/Qualifiers
     source          1..4407
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..327
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             349..366
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             379..399
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgagcgata aaattattca cctgactgac gacagttttg acacggatgt actcaaagcg
       61 gacggggcga tcctcgtcga tttctgggca gagtggtgcg gtccgtgcaa aatgatcgcc
      121 ccgattctgg atgaaatcgc tgacgaatat cagggcaaac tgaccgttgc aaaactgaac
      181 atcgatcaaa accctggcac tgcgccgaaa tatggcatcc gtggtatccc gactctgctg
      241 ctgttcaaaa acggtgaagt ggcggcaacc aaagtgggtg cactgtctaa aggtcagttg
      301 aaagagttcc tcgacgctaa cctggccgga tctggcagtg gttctggtca tcaccatcac
      361 catcactccg cgggtagcga gaatctttat tttcagggcg ccatggaaaa accgtacacg
      421 attggactag acataggaac aaactcggtt ggatgggcag tgttaacaga tcaatatgat
      481 ttagtgaaga ggaaaatgaa aattgctgga gactctgaga aaaaacagat aaagaaaaat
      541 ttctggggag ttagactatt tgatgagggt caaacggcag ctgatagaag aatgactaga
      601 acagctagaa ggagaataga gagaagacgc aatcgtattt cgtatttaca agggatattt
      661 gcggaagaaa tgtctaaggt ggatgcaaat ttcttttgcc gattaagtga tagcttttat
      721 gttgataacg aaaaaagaaa tagtcgtcat cctttttttg cgactataga ggaagaagta
      781 gaataccaca aaaactaccc aaccatttat cacctgagag aagaactcgt taattcttca
      841 gaaaaagctg atttaagatt agtttattta gctttagctc atattataaa atatcgaggg
      901 aactttttaa ttgagggagc attagatacc caaaatactt ctgttgatgg aatatataag
      961 caatttatac aaacatataa tcaagtgttt gcaagtggca ttgaagacgg ttcgctaaaa
     1021 aaattggaag ataataagga cgtagctaaa attctcgttg aaaaagttac ccgaaaagaa
     1081 aaacttgaga gaattttaaa attataccct ggtgaaaaac ctgccggaat gtttgctcag
     1141 tttatcagct tgattgtagc cagtaaaggg aattttcaaa agccttttga tcttattgag
     1201 aaatcggata ttgaatgtgc gaaagatagc tatgaagaag atttagagtc tttgttagcg
     1261 ctaattggag atgaatatgc ggaacttttt gttgcagcga aaaatgcata tagtgctgtg
     1321 gtgttatcta gtattatcac agtggctgag gctgaaacaa acgcgaagtt atccgcaagt
     1381 atgattgaac gttttgatac acatgaagaa gatttagggg aattgaaagc atttatcaag
     1441 ctccatcttc ctaaacacta tgaagaaata tttagtaata ccgaaaaaca tggttacgca
     1501 ggttatatag acggtaaaac aaagcaggca gacttctata aatacatgaa aacaacatta
     1561 gagaacattg aaggccccga ttattttatt gctaaaatag aaaaagaaaa ctttttacga
     1621 aaacaacgaa cctttgataa tggggctatc ccgcatcaat tacatttaga agaattagaa
     1681 gctattttac accagcaagc aaaatattat ccttttttaa aagaaaacta tgataaaatc
     1741 aaaagtttag taacttttag aattccatat tttgttggtc cgctagctaa cggacagagc
     1801 gaattcgcat ggttaacaag aaaagctgat ggtgagatta gaccgtggaa tatagaagag
     1861 aaagtggatt tcggtaagtc agcggtcgat ttcattgaaa agatgaccaa taaagataca
     1921 tatttaccta aagaaaatgt attgcctaaa cacagtctct gttatcaaaa atatttggtt
     1981 tataatgagt taaccaaagt tagatacatt aatgaccaag ggaagactag ctatttttct
     2041 gggcaggaaa aagaacagat ttttaatgac ttatttaagc aaaagcgtaa agttaaaaag
     2101 aaggatttag agctgttctt acgcaatatg agccatgttg aaagtccaac tattgaaggc
     2161 ctggaagact cgtttaattc aagttattct acctatcatg atttactcaa ggtagggata
     2221 aaacaagaaa tccttgataa cccagtaaat acggaaatgc tagaaaatat agtcaaaatt
     2281 ttaactgtgt ttgaagataa acgtatgatt aaagagcagt tacagcaatt ttctgatgtt
     2341 ttagatgggg ttgttttgaa aaaattagaa cgacgacatt atacaggatg gggaaggtta
     2401 tcagctaagt tattaatggg aattcgtgat aagcaatctc acttaactat tttagattat
     2461 ttgatgaatg atgatgggct taatcgtaat ctgatgcaac ttataaatga cagtaattta
     2521 tcttttaaat caataattga aaaagaacaa gtaactacag cagataaaga tattcaaagt
     2581 attgtggcag accttgctgg tagcccggct ataaaaaaag gtattttaca aagcttgaaa
     2641 attgtcgatg aacttgttag tgtaatgggc tacccacctc aaactattgt tgtggaaatg
     2701 gcaagagaaa atcaaacaac aggaaaagga aaaaataatt ctagaccacg ttataaatca
     2761 ttagaaaaag cgattaaaga atttggtagt caaattctaa aagagcatcc gactgataat
     2821 caagaattaa gaaataatag attgtattta tattatttgc aaaatggtaa ggatatgtat
     2881 actggacaag aattagatat tcataatctt tctaattatg atattgatca tattgtgcca
     2941 cagagtttta taacagataa ctccgttgat aatcttgtgt taacaagttc ggctgggaat
     3001 cgggaaaaag gggatgatgt tccaccacta gagatcgttc gaaagagaaa agtattctgg
     3061 gagaagttat accaagggaa tttgatgagt aaacgtaaat ttgattattt aactaaagct
     3121 gaacctggtg gtcttacaga accggataaa gcgagattta ttcatcggca attagtggaa
     3181 acccgtcaaa taacgaagaa tgtagctaat attttacatc aacgttttaa ttatgaaaaa
     3241 gacgatcatg ggaatactat gaaccaagtt cggattgtaa cattaaaatc tgcactggtt
     3301 agccaattcc gtaagcaatt tcaattgtat aaggtgcgtg gagttaatga ttatcaccac
     3361 gctcatgatg cttatttaaa tggggtagtc gcgaatacgt tattaaaagt atatcctcaa
     3421 ttagagccgg aatttgttta cggcgattac catcaatttg attggtttaa agcgaataaa
     3481 gctacggcga aaaaacaatt ttatacaaac atcatgttat tctttgcgca aaaagatcga
     3541 ataatagatg aaaacggtga aattctttgg gataaaaaat atttggatac tgtcaaaaaa
     3601 gtcatgagtt atcgacaaat gaatattgtt aagaaaacag aaattcaaaa aggagaattt
     3661 tcaaaagcta caattaagcc caaaggaaat tccagtaaat taattcctag aaaaactaat
     3721 tgggacccga tgaaatatgg tggtcttgat agccctaata tggcatatgc agtggttatt
     3781 gaatatgcaa aagggaaaaa taaattagtt ttcgagaaaa aaattatccg tgttactatc
     3841 atggaacgta aagcatttga aaaagatgag aaagcctttt tagaagaaca aggttatcgt
     3901 cagcctaaag tcctcgcgaa attaccgaaa tatacgttat atgaatgtga ggaaggacgt
     3961 cggagaatgc tggctagtgc taatgaagct cagaaaggaa atcagcaggt attgccaaac
     4021 cacctggtaa cattactaca ccatgcggca aattgtgagg caagtgatgg gaaaagcctt
     4081 gattatattg aaagtaatag agaaatgttt gctgaactac tagcgcacgt atcagaattt
     4141 gccaaaagat atacgttagc tgaagctaat ctaaacaaaa ttaatcagct ttttgagcaa
     4201 aataaagaag gtgacatcaa ggcaattgca cgatcttttg ttgatttaat ggcattcaat
     4261 gcgatgggtg ctccggcaag ttttaaattt tttgaaacca ctattgagcg taaaagatat
     4321 aacaatctaa aagaactatt aagctccacc ataatctacc aatcaatcac aggactatat
     4381 gaatcacgaa aaaggctgga tgactaa
//
