LOCUS       sequence-100654-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of GFP, rtTA and 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-GP
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..78
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(79..696,697..702,703..738,739..741,742..777,778..780,
                     781..816,817..822)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             829..885
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(886..905)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gagctgtaca agggcagtgg agctactaac ttcagcctgc tgaagcaggc tggtgacgtc
       61 gaggagaatc ctggccccat gtctagactg gacaagagca aagtcataaa cggcgctctg
      121 gaattactca atggagtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg
      181 ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc
      241 ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca
      301 tggcaagact ttctgcggaa caacgccaag tcattccgct gtgctctcct ctcacatcgc
      361 gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa
      421 aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg
      481 tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca
      541 aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt
      601 gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata
      661 tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cggccgacgc ccttgacgat
      721 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
      781 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtccggcgc tactaacttc
      841 agcctgctga agcaggctgg tgacgtcgag gagaatcctg gtcccatgac cgagtacaag
      901 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
      961 gcgttcgccg acta
//
