LOCUS       sequence-100655-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of GFP, rtTA and 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-GP
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..31
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             83..139
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(140..757,758..763,764..799,800..802,803..838,839..841,
                     842..877,878..883)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
       61 cgagctgtac aagggcagtg gagctactaa cttcagcctg ctgaagcagg ctggtgacgt
      121 cgaggagaat cctggcccca tgtctagact ggacaagagc aaagtcataa acggcgctct
      181 ggaattactc aatggagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
      241 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
      301 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
      361 atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc tctcacatcg
      421 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
      481 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
      541 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
      601 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
      661 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
      721 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggccgacg cccttgacga
      781 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
      841 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtccggcg ctactaactt
      901 cagcctgctg aagcaggct
//
